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Gangliosides are ubiquitous membrane components in mammalian cells and are staggested to play important roles in various functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. Among all ganglioseries gangliosides, CM3 has the simplest carbohydrate structure, and has been shown as a major Banglioside in male reproductive system. To study GM3 distribution in the seminiferous tubule and epididymis, frozen sections were stained with specific monoclonal antibody (MAB) against ganglioside GM3. In the seminiferous tubule of testis, pachytene spermatocytes and spermmatids expressed ganglioside GM3, but not in spermatogonia and sertoli cells. Spermatogonia and sertoli cells near the basement membrane were negatively reacted to anti-GM3. In the epididymis, GM3 was expressed only in some interstitial cells. Taken together, these results suggest that the expression of ganglioside GM3 in rat seminiferous tubule and epididymis is spatio- temporally regulated during spermatogenesis.
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